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housekeeping protein β tubulin  (MedChemExpress)


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    Structured Review

    MedChemExpress housekeeping protein β tubulin
    Housekeeping Protein β Tubulin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/housekeeping+protein+%CE%B2+tubulin/Beta+Tubulin+Antibody/pmc12623988-261-13-30
    Average 93 stars, based on 7 article reviews
    housekeeping protein β tubulin - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Candida albicans enhances melanoma cell aggressiveness through p38-MAPK and HIF-1α pathways and metabolic reprogramming
    Article Snippet: Membranes were then blocked for 2 h with Tris Buffered Saline Milk (TBSM; 50 mM tris-HCl pH 7.5, 150 mM NaCl, 0.1% (v/v) Tween 20 and 5% (w/v) low fat dried milk, both from Sigma-Aldrich, MO, USA). .. Rabbit monoclonal antibodies against c-Fos (YA506, HY-P80081) and the housekeeping protein β-Tubulin (HY-P80487) diluted 1:1,000 in TBSM were used as primary antibodies (MedChemExpress, NJ, USA) and incubated with the membrane overnight at 4°C. .. After three 5 min washes with TBS, goat anti-rabbit IgG (Invitrogen, Thermo Fisher Scientific) diluted 1:5,000 in TBSM was used as secondary antibody and incubated with the membrane for 30 min. All the steps were carried out at room temperature and agitation unless otherwise indicated.

    Article Title: Enhancement of melanoma aggressiveness via p38-MAPK, HIF-1α pathways, and metabolic reprogramming induced by Candida albicans
    Article Snippet: Membranes were then blocked for 2 h with Tris Buffered Saline Milk (TBSM; 50 mM tris-HCl pH 7.5, 150 mM NaCl, 0.1% (v/v) Tween 20 and 5% (w/v) low fat dried milk, both from Sigma-Aldrich). .. Rabbit monoclonal antibodies against c-Fos (YA506, Cat. # HY- P80081 ) and the housekeeping protein β-Tubulin (Cat. # HY- P80487 ) diluted 1:1,000 in TBSM were used as primary antibodies (MedChemExpress) and incubated with the membrane overnight at 4 °C. .. After three 5 min washes with TBS, goat anti-rabbit IgG (Thermo Fisher Scientific, Cat. # 31460) diluted 1:5,000 in TBSM was used as secondary antibody and incubated with the membrane for 30 min. All the steps were carried out at room temperature and agitation unless otherwise indicated.

    Incubation:

    Article Title: Candida albicans enhances melanoma cell aggressiveness through p38-MAPK and HIF-1α pathways and metabolic reprogramming
    Article Snippet: Membranes were then blocked for 2 h with Tris Buffered Saline Milk (TBSM; 50 mM tris-HCl pH 7.5, 150 mM NaCl, 0.1% (v/v) Tween 20 and 5% (w/v) low fat dried milk, both from Sigma-Aldrich, MO, USA). .. Rabbit monoclonal antibodies against c-Fos (YA506, HY-P80081) and the housekeeping protein β-Tubulin (HY-P80487) diluted 1:1,000 in TBSM were used as primary antibodies (MedChemExpress, NJ, USA) and incubated with the membrane overnight at 4°C. .. After three 5 min washes with TBS, goat anti-rabbit IgG (Invitrogen, Thermo Fisher Scientific) diluted 1:5,000 in TBSM was used as secondary antibody and incubated with the membrane for 30 min. All the steps were carried out at room temperature and agitation unless otherwise indicated.

    Article Title: Enhancement of melanoma aggressiveness via p38-MAPK, HIF-1α pathways, and metabolic reprogramming induced by Candida albicans
    Article Snippet: Membranes were then blocked for 2 h with Tris Buffered Saline Milk (TBSM; 50 mM tris-HCl pH 7.5, 150 mM NaCl, 0.1% (v/v) Tween 20 and 5% (w/v) low fat dried milk, both from Sigma-Aldrich). .. Rabbit monoclonal antibodies against c-Fos (YA506, Cat. # HY- P80081 ) and the housekeeping protein β-Tubulin (Cat. # HY- P80487 ) diluted 1:1,000 in TBSM were used as primary antibodies (MedChemExpress) and incubated with the membrane overnight at 4 °C. .. After three 5 min washes with TBS, goat anti-rabbit IgG (Thermo Fisher Scientific, Cat. # 31460) diluted 1:5,000 in TBSM was used as secondary antibody and incubated with the membrane for 30 min. All the steps were carried out at room temperature and agitation unless otherwise indicated.

    Membrane:

    Article Title: Candida albicans enhances melanoma cell aggressiveness through p38-MAPK and HIF-1α pathways and metabolic reprogramming
    Article Snippet: Membranes were then blocked for 2 h with Tris Buffered Saline Milk (TBSM; 50 mM tris-HCl pH 7.5, 150 mM NaCl, 0.1% (v/v) Tween 20 and 5% (w/v) low fat dried milk, both from Sigma-Aldrich, MO, USA). .. Rabbit monoclonal antibodies against c-Fos (YA506, HY-P80081) and the housekeeping protein β-Tubulin (HY-P80487) diluted 1:1,000 in TBSM were used as primary antibodies (MedChemExpress, NJ, USA) and incubated with the membrane overnight at 4°C. .. After three 5 min washes with TBS, goat anti-rabbit IgG (Invitrogen, Thermo Fisher Scientific) diluted 1:5,000 in TBSM was used as secondary antibody and incubated with the membrane for 30 min. All the steps were carried out at room temperature and agitation unless otherwise indicated.

    Article Title: Enhancement of melanoma aggressiveness via p38-MAPK, HIF-1α pathways, and metabolic reprogramming induced by Candida albicans
    Article Snippet: Membranes were then blocked for 2 h with Tris Buffered Saline Milk (TBSM; 50 mM tris-HCl pH 7.5, 150 mM NaCl, 0.1% (v/v) Tween 20 and 5% (w/v) low fat dried milk, both from Sigma-Aldrich). .. Rabbit monoclonal antibodies against c-Fos (YA506, Cat. # HY- P80081 ) and the housekeeping protein β-Tubulin (Cat. # HY- P80487 ) diluted 1:1,000 in TBSM were used as primary antibodies (MedChemExpress) and incubated with the membrane overnight at 4 °C. .. After three 5 min washes with TBS, goat anti-rabbit IgG (Thermo Fisher Scientific, Cat. # 31460) diluted 1:5,000 in TBSM was used as secondary antibody and incubated with the membrane for 30 min. All the steps were carried out at room temperature and agitation unless otherwise indicated.



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